Editing Mycoplasma Assay Protocol (section)

=Required Reagents=

Before we begin, you will need to ensure that you have access to the following reagents and equipment:

{| class="wikitable"
|+Reagents
|-
! scope="col"| Item
! scope="col"| Location
|-
|'''Lucetta 2 Luminometer'''
|For our purposes, this is found in the ''Nathanson Lab'' at '''CHS 23-376''', although you will likely need someone from the ''Nathanson Lab'' main room to let you in.
|-
| '''Secondary carrying container for bioharzardous materials'''
| You can borrow one from the ''Nathanson Lab'', or use your own. Both will need to be sterilized with 70% isopropyl alcohol after use.
|-
| '''Cell culture media'''
| Varies.
|-
| '''96 Well microtiter plate'''
| Bring your own, or use the one in the ''Nathanson Lab'' for ongoing bioassays.
|-
| '''100 µL pipette'''
| Lab Benchtop.
|-
| '''100 µL pipette tips'''
| Lab Benchtop.
|-
| '''100 µL of UltraPure H2O (or buffer) for a control sample'''
| Lab Benchtop.
|-
| '''MycoAlert Substrate'''
| In MycoAlert Kit, '''Cat #: LT07-318'''. Stored in the Lai lab in the -80ºC freezer as 310 µL aliquots, which is good for 3 samples. Note that aliquots should not be freeze/thawed, so discard any excess once you’re finished. 
|-
| '''MycoAlert Reagent'''
| In MycoAlert Kit, '''Cat #: LT07-318'''. Stored in the Lai lab in the -80ºC freezer as 310 µL aliquots, which is good for 3 samples. Note that aliquots should not be freeze/thawed, so discard any excess once you’re finished. 
|}