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Mycoplasma Assay Protocol
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====Preparing the Assay==== # Head over to the Nathanson Lab main room. It is located at '''CHS 23-234'''. You should contact someone ahead of time. Otherwise, any '''Lucetta 2 Luminometer''' will do. # Next, '''take out a microtiter plate''' designated for biohazardous samples. The ''Nathanson Lab'' usually keeps a few in use in the middle drawer on the first lab bench once you enter the room. These microtiter plates are often used for assays involving mouse blood, so treat them as biohazardous. You should do the same if you use your own plate. # In the microtiter plate, '''place 100 µL of sample (or control) into unused wells'''. # Place the plate in your biohazardous secondary container for transport. # Go to the assay room at '''CHS 23-376'''. A member of the ''Nathanson Lab'' will likely need to accompany you to let you in and log you into the machine. # To each sample, '''add 100 µL of thawed MycoAlert Reagent''' (labeled “R” on aliquots) and '''wait 5 minutes'''.
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