Editing Nanopore RNA Sequencing Protocol (section)

====Samtools====

'''Samtools''' is a suite of programs that can be used in Linux and Mac to further process high-throughput DNA / RNA sequencing data. It’s name is a reference to the <code>.SAM</code> file, which stands for “'''S'''equence '''A'''lignment '''M'''ap format”, a precursor to the binary version of this file (the <code>.BAM</code> file). We will use ''Samtools'' to modify our aligned sequencing data prior to m6A modification detection, so you should have it pre-installed before trying to analyze any of your data.

''Samtools'' can be installed via ''Conda'':

<code>Bash</code>
<syntaxhighlight lang="Bash">
conda install -c bioconda samtools
</syntaxhighlight>