Below is a list of all experimental records and data. Records typically include a sample ID as well as a link to files for that run. These files are stored on a google drive that will require access in order to download. All records here are backed up on the lab drive in their respective project folders as well.

M6A G-RAMP

Below is a sortable table for all Nanopore m6A RNA runs conducted in the Lai Lab. These data are also stored on the lab drive in addition to a google drive backup.

Entry	Sample ID (Notebook Reference)	Protocol	Cell Line	IDH Status	Output	Description / Notes	Date of Run
10	AQ45U8W1123 (LLT-001p87)	Nanopore RNA Sequencing Protocol	U87	WT	Good	81k reads run. This was freshly extracted mRNA, no rRNA cleanup step. Differences in this run: mRNA input was checked for 2.0 or greater ratio in 260/230 and 260/280 regions. mRNA input was the full 9 µL, for around 333 ng of mRNA total. I also turned the pellet fast during ethanol wash, pipetted instead of flicked the tube, and eluted twice. These changes were added to the protocol.	11/09/23
9	FC14U8W723 (LLT-001p71-72)	Nanopore RNA Sequencing Protocol	U87	WT	Good	56k reads run. This was a test run using stored U87 cell RNA that had been purified for mRNA prior to the run. This run generated a decent output.	07/14/23
8	FC31HKW623 (LLT-001p67)	Nanopore RNA Sequencing Protocol	HK217	WT	Okay	This was a control run, which mostly contained synthetic RNA oligos containing Gluc/Cluc and m6A modifications. Run on a flongle flow cell. HK217 IDH-WT spiked in as a control.	06/14/23
7	FC92HKW623 (LLT-001p63)	Nanopore RNA Sequencing Protocol	HK217	WT	Poor	This was the first attempt at a control run, which mostly contained synthetic RNA oligos containing Gluc/Cluc and m6A modifications. Run on a flongle flow cell. HK217 IDH-WT spiked in as a control.	06/02/23
6	FC04HKW323 (LLT-001p24-25)	Nanopore RNA Sequencing Protocol	HK217	WT	Good	57k reads. This run was a decent run using a Flongle Flow Cell and a gliomasphere cell line.	03/16/23
5	FR56U8W1221	Nanopore RNA Sequencing Protocol	U87	WT	Good	This was the vector control U87 re-run by Terry Prins and Sean Pianka. This run yielded a good throughput.	12/20/21
4	FQ15U8W921	Nanopore RNA Sequencing Protocol	U87	WT	Poor	This is a vector control U87 sample run by Bryan Kevan and Sean Pianka. This run yielded a very low amount of reads before failing.	09/17/21
3	FO96U8W521	Nanopore RNA Sequencing Protocol	U87	WT	Poor	This is a vector control U87 sample run by Bryan Kevan and Sean Pianka. This run did not yield an optimal amount of reads.	05/05/21
2	FM31U8M720	Nanopore RNA Sequencing Protocol	U87	Mut	Good	This sample was a U87 sample run by Bryan Kevan and Sean Pianka.	07/15/20
1	FM68U8W720	Nanopore RNA Sequencing Protocol	U87	WT	Poor	This is a vector control U87 sample run by Bryan Kevan and Sean Pianka. This run did not yield an optimal amount of reads.	07/14/20