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Nanopore RNA Sequencing Protocol
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===R File Paths=== <code>R</code> <syntaxhighlight lang="R"> SAMPLEID="FAR91556" GREFERENCEID="GRCh38.p14.rna.fna" CHR="chr19" OUTPUT_PATH=paste("/home/tj/Research/Data/Nanopore/Aligned", SAMPLEID, "", sep = "/") M6ANET_PATH=paste("/home/tj/Research/Data/Nanopore/m6ANet_results", SAMPLEID, "", sep = "/") </syntaxhighlight> Note that at any time, you can check these in Terminal using the <code>echo</code> command. For example, if I wanted to check what my current <code>SAMPLEID</code> was, I would type <code>echo "${SAMPLEID}"</code> and execute. Also, notice that here we are only defining one sample to be analyzed, which is all we need for ''m6ANet'' or ''EpiNano-SVM''. ''EpiNano-Error'', however, requires two samples as input if you use it. For that analysis, I will leave defining the samples to be compared at the start of the ''EpiNano-Error'' section.
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